犬におけるメチシリン耐性ブドウ球菌の起源解明
メンバー： 石原加奈子
分野： 動物生命科学、基礎医学、生物科学
所属： 農学研究院 動物生命科学部門
キーワード： メチシリン耐性、 ブドウ球菌、 分子疫学、 犬
ウェブサイト：
研究概要
近年、メチシリン耐性Staphylococcus pseudintermedius (MRSP) の出現が報告され、犬において増加している。遺伝子型別により、欧州では、sequence type (ST)71-spa t02-SCCmec II–IIIが、北米では ST68-spa t06-SCCmec Vが流行していることが知られている。
本研究では、日本の犬由来MRSPの遺伝学的特徴を明らかにし、欧州、北米の流行株と比較し、日本のMRSPの起源を検索した。
主要論文・参考事項
1) Ishihara, K., Saito, M., Shimokubo, N., Muramatsu, Y., Maetani, S., Tamura, Y., 2014. Epidemiological analysis of methicillin-resistant Staphylococcus aureus carriage among veterinary staff of companion animals in Japan. J Vet Med Sci 76, 1627-1629.
2) Ishihara, K., Saito, M., Shimokubo, N., Muramatsu, Y., Maetani, S., Tamura, Y., 2014. Methicillin-resistant Staphylococcus aureus carriage among veterinary staff and dogs in private veterinary clinics in Hokkaido, Japan. Microbiol Immunol 58, 149-154.
3) Ishihara, K., Takahashi, R., Andoh, M., Makita, K., Kamiji, S., Ueno, H., Muramatsu, Y., Tamura, Y., 2012. Effects of climatic elements on Campylobacter-contaminated chicken products in Japan. Epidemiol Infect 140, 991-996.
4) Ishihara, K., Hosokawa, Y., Makita, K., Noda, J., Ueno, H., Muramatsu, Y., Mukai, T., Yamamoto, H., Ito, M., Tamura, Y., 2012. Factors associated with antimicrobial-resistant Escherichia coli in zoo animals. Res Vet Sci 93, 574-580.
5) Ishihara, K., Takahashi, R., Andoh, M., Ueno, H., Muramatsu, Y., Tamura, Y., 2012. Seasonal variation in Campylobacter-contaminated retail chicken products: a year-round investigation in Japan. J Vet Med Sci 74, 117-120.
6) Ishihara, K., Shimokubo, N., Sakagami, A., Ueno, H., Muramatsu, Y., Kadosawa, T., Yanagisawa, C., Hanaki, H., Nakajima, C., Suzuki, Y., Tamura, Y., 2010. Occurrence and molecular characteristics of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in an academic veterinary hospital. Appl Environ Microbiol 76, 5165-5174.
お問い合わせ先
東京農工大学・先端産学連携研究推進センター
urac[at]ml.tuat.ac.jp（[at]を@に変換してください）
Studies on the origins of methicillin-resistant staphylococci on dogs
Research members： Dr. Kanako Ishihara
Research fields： Animal life science, Basic medicine, Biological Science
Departments： Section of Animal Life Science, Institute of Agriculture
Keywords： methicillin resistance, staphylococci, molecular epidemiology, dog
Web site：
Summary
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains have recently been reported, and are increasingly being isolated from dogs. Molecular analysis of MRSP isolates revealed that sequence type (ST)71-spa t02-SCCmec II–III and ST68-spa t06-SCCmec V are the major MRSP clones in Europe and North America, respectively.
To compare the characteristics of MRSP isolates from Japan with those from Europe, North America, and other countries, the current study examined canine MRSP isolates from Japan using MLST and some molecular epidemiological techniques.
All MRSP SCCmec II–III isolates were thought to belong to one clonal lineage that had spread worldwide. However, in this study we confirmed that in Japan, SCCmec II–III elements are present in three related clonal lineages: ST71, ST169, and ST354.
Reference articles and patents
1) Ishihara, K., Saito, M., Shimokubo, N., Muramatsu, Y., Maetani, S., Tamura, Y., 2014. Epidemiological analysis of methicillin-resistant Staphylococcus aureus carriage among veterinary staff of companion animals in Japan. J Vet Med Sci 76, 1627-1629.
2) Ishihara, K., Saito, M., Shimokubo, N., Muramatsu, Y., Maetani, S., Tamura, Y., 2014. Methicillin-resistant Staphylococcus aureus carriage among veterinary staff and dogs in private veterinary clinics in Hokkaido, Japan. Microbiol Immunol 58, 149-154.
3) Ishihara, K., Takahashi, R., Andoh, M., Makita, K., Kamiji, S., Ueno, H., Muramatsu, Y., Tamura, Y., 2012. Effects of climatic elements on Campylobacter-contaminated chicken products in Japan. Epidemiol Infect 140, 991-996.
4) Ishihara, K., Hosokawa, Y., Makita, K., Noda, J., Ueno, H., Muramatsu, Y., Mukai, T., Yamamoto, H., Ito, M., Tamura, Y., 2012. Factors associated with antimicrobial-resistant Escherichia coli in zoo animals. Res Vet Sci 93, 574-580.
5) Ishihara, K., Takahashi, R., Andoh, M., Ueno, H., Muramatsu, Y., Tamura, Y., 2012. Seasonal variation in Campylobacter-contaminated retail chicken products: a year-round investigation in Japan. J Vet Med Sci 74, 117-120.
6) Ishihara, K., Shimokubo, N., Sakagami, A., Ueno, H., Muramatsu, Y., Kadosawa, T., Yanagisawa, C., Hanaki, H., Nakajima, C., Suzuki, Y., Tamura, Y., 2010. Occurrence and molecular characteristics of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in an academic veterinary hospital. Appl Environ Microbiol 76, 5165-5174.
Contact
University Research Administration Center(URAC),
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
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