ウイルス感染の制御分子機構を活用した新規病害抵抗性植物の開発
メンバー： 佐々木信光
分野： 生体分子科学、生物科学、生産環境農学
所属： その他
キーワード： 植物のウイルス抵抗性、 plant virus resistance、遺伝子制御、 gene regulation、遺伝子組換え植物、 genetically modified plants
研究概要
植物は、動物とは異なるタイプの免疫機構を備えており、ウイルスの侵入に対して様々な防御反応を起こし、ウイルスの感染拡大を阻止しています。一方で、ウイルスは、植物からの防御反応を回避しつつ、植物がもつウイルス増殖補助因子（ウイルス感受性因子）を利用して、ウイルスは植物全体へと感染を拡大させています。我々は、以下のような研究テーマについて、ウイルス抵抗性機構およびウイルス増殖機構の両側面から、植物とウイルス間の相互作用を分子レベルで解析し、広範囲のウイルスに対応可能な新規病害抵抗性植物の開発を目指しています。
①タバコのウイルス抵抗性遺伝子Nの発現制御およびNを介した細胞死誘導の分子機構の解明
②ウイルス移行タンパク質と相互作用する植物遺伝子産物の機能解析
③ウイルス感染に関与する抵抗性・感受性関連遺伝子を利用した遺伝子組換え抵抗性植物の開発
主要論文・参考事項
1. Sasaki N, Takanoka M, Sasaki S, Hirai K, Meshi T, Nyunoya H. The splice variant Ntr encoded by the tobacco resistance gene N has a role for negative regulation of antiviral defense responses. Physiol. Mol. Plant Pathol. 84: 92-98. (2013)
2. Takano M, Haque MA, Odaira S, Nakata K, Sasaki N, Nyunoya H. Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the tobacco mosaic virus resistance gene N and defense-related responses including ROS production. Plant Biotechnol. 30: 37-46. (2013)
3. Sasaki N, Odawara T, Deguchi M, Nyunoya H. Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris. Physiol. Mol. Plant Pathol. 78: 38-44. (2012)
4. Sasaki N, Odawara T, Deguchi M, Matsushita Y, Nyunoya H. Interference with Tomato mosaic virus cell-to-cell movement by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species. J. Gen. Plant Pathol. 76: 69-73. (2010)
5. Harries P, Park J-W, Sasaki N, Ballard K, Maule A, Nelson R. Differing requirements for actin and myosin by plant viruses for sustained intercellular movement. Proc Natl Acad Sci USA. 106: 17594-17599. (2010)
お問い合わせ先
東京農工大学・先端産学連携研究推進センター
urac[at]ml.tuat.ac.jp（[at]を@に変換してください）
Development of novel disease resistance plants by utilization of molecular mechanisms that regulate virus infection
Research members： Nobumitsu Sasaki PhD.
Research fields： Biomolecular science, Biological Science, Plant production and environmental agriculture
Departments： Department, Center
Keywords： plant virus resistance,gene regulation,genetically modified plants
Summary
Plants have distinct immune mechanisms from those of animals and induce various defense responses against virus invasion, preventing expansion of virus infection. On the other hand, viruses can spread throughout the whole plant by escaping from plant defense responses and also utilizing plant factors that rather help viruses amplify. In order to develop novel resistance plants that can cover a wide range of viruses, we have been doing molecular-level studies on plant-virus interactions in the processes of both virus resistance and virus amplification. Our current research themes are as follows.
i)Study on molecular mechanisms of the regulation of virus resistance gene N and N-mediated cell death induction
ii)Functional analysis on plant gene products that interacts with virus movement protein
iii)Development of novel virus resistance plants by genetically engineering resistance/susceptibility-related plant genes involved in virus infection
Reference articles and patents
1. Sasaki N, Takanoka M, Sasaki S, Hirai K, Meshi T, Nyunoya H. The splice variant Ntr encoded by the tobacco resistance gene N has a role for negative regulation of antiviral defense responses. Physiol. Mol. Plant Pathol. 84: 92-98. (2013)
2. Takano M, Haque MA, Odaira S, Nakata K, Sasaki N, Nyunoya H. Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the tobacco mosaic virus resistance gene N and defense-related responses including ROS production. Plant Biotechnol. 30: 37-46. (2013)
3. Sasaki N, Odawara T, Deguchi M, Nyunoya H. Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris. Physiol. Mol. Plant Pathol. 78: 38-44. (2012)
4. Sasaki N, Odawara T, Deguchi M, Matsushita Y, Nyunoya H. Interference with Tomato mosaic virus cell-to-cell movement by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species. J. Gen. Plant Pathol. 76: 69-73. (2010)
5. Harries P, Park J-W, Sasaki N, Ballard K, Maule A, Nelson R. Differing requirements for actin and myosin by plant viruses for sustained intercellular movement. Proc Natl Acad Sci USA. 106: 17594-17599. (2010)
Contact
University Research Administration Center(URAC),
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
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