皮膚バリア機能の異常に関わる哺乳動物の皮膚疾患の研究
メンバー： 西藤公司
分野： 動物生命科学、内科系臨床医学
所属： 農学研究院
キーワード： 皮膚科学、 角層、皮膚バリア機能、ブドウ球菌、アトピー性皮膚炎
ウェブサイト：
研究概要
皮膚は生体の最も外側に存在し、異物や病原体が外界から生体内へと侵入するのを防ぐバリアとしての役割をもっています。この皮膚のバリア機能は、主に皮膚の最外層を覆う角層、ならびに角層の下で強固に接着し合う表皮角化細胞により成り立っています。当研究室では哺乳動物に発症する各種皮膚疾患と、角層バリア機能の異常との関連についての解明を試みています。
１．膿皮症におけるブドウ球菌の経皮侵入機構について
膿皮症はヒト、犬、馬などに認められる細菌性皮膚感染症で、本症の病変部からはブドウ球菌が高率に分離されます。当研究室ではブドウ球菌が、どのような機序で哺乳動物の皮膚バリアを突破しながら表皮や毛包上皮へと侵入するのかを動物モデルを用いて解析しています。特に膿皮症の発症に関与する角層バリアの異常と、ブドウ球菌が産生する表皮剥脱毒素の病的関与について解析を進めています。
２．アトピー性皮膚炎における角層バリアの異常について
アトピー性皮膚炎は慢性・反復性を示すそう痒性皮膚疾患であり、ヒトのみならず犬においても発症することが知られています。当研究室では犬アトピー性皮膚炎の症例における角層バリア関連分子の発現異常について解析すると共に、その発現異常と本症の病態との関与について解析を行っています。
主要論文・参考事項
1. Yoon JS, Nishifuji K, Ishioroshi S, Ide K, Iwasaki T.Skin lipid profiling in normal and seborrhoeic shih tzu dogs.Vet Dermatol. 2013; 24(1):84-89.
2. Nishifuji K, Yoon JS. The stratum corneum: the rampart of the mammalian body.Vet Dermatol. 2013; 24(1):60-72.
3. Kanda S, Sasaki T, Shiohama A, Nishifuji K, Amagai M, Iwasaki T, Kudoh J. Characterization of canine filaggrin: gene structure and protein expression in dog skin.Vet Dermatol. 2013; 24(1):25-31.
4. Yoon JS, Nishifuji K, Sasaki A, Ide K, Ishikawa J, Yoshihara T, Iwasaki T. Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis.Exp Dermatol. 2011; 20(9):732-736.
5. Iyori K, Futagawa-Saito K, Hisatsune J, Yamamoto M, Sekiguchi M, Ide K, Son WG, Olivry T, Sugai M, Fukuyasu T, Iwasaki T, Nishifuji K. Staphylococcus pseudintermedius exfoliative toxin EXI selectively digests canine desmoglein 1 and causes subcorneal clefts in canine epidermis.Vet Dermatol. 2011; 22(4):319-326.
6. Iyori K, Hisatsune J, Kawakami T, Shibata S, Murayama N, Ide K, Nagata M, Fukata T, Iwasaki T, Oshima K, Hattori M, Sugai M, Nishifuji K. Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs.FEMS Microbiol Lett. 2010; 312(2):169-175.
7. Shimada K, Yoon JS, Yoshihara T, Iwasaki T, Nishifuji K. Increased transepidermal water loss and decreased ceramide content in lesional and non-lesional skin of dogs with atopic dermatitis.Vet Dermatol. 2009; 20(5-6):541-546.
8. Nishifuji K, Sugai M, Amagai M. Staphylococcal exfoliative toxins: "molecular scissors" of bacteria that attack the cutaneous defense barrier in mammals.J Dermatol Sci. 2008; 49(1):21-31.
お問い合わせ先
東京農工大学・先端産学連携研究推進センター
urac[at]ml.tuat.ac.jp（[at]を@に変換してください）
Investigation of pathomechanisms of skin diseases associated with aberrant cutaneous barrier function in mammals
Research members： Dr. Koji Nishifuji
Research fields： Animal life science, Clinical internal medicine
Departments： Institute of Agriculture
Keywords： dermatology, stratum corneum, cutaneous barrier function, Staphylococcus, atopic dermatitis
Web site：
Summary
Skin is an external organ that covers the exterior surface of mammalian body, and plays the most important role in protecting the internal organ against the entry of pathogen or foreign bodies. The barrier function of the skin is mainly composed of cornified layer, the outermost layer of the skin, as well as strong cohesion between adjacent epidermal keratinocytes. Our laboratory is aiming at investigating the relationship between skin diseases in mammals and aberrant cutaneous barrier functions.
1. Mechanisms of bacterial cutaneous invasion in staphylococcal pyoderma
Pyoderma is a group of bacterial infectious diseases commonly recognized in humans, dogs and horses. In the skin lesions of pyoderma, Staphylococcus spp. is frequently isolated. Our laboratory is investigating how staphylococci break cutaneous barrier function and invate into the epidermis or hair follicle epithelia. Especially, we are aiming at investigating detailed mechanisms of aberrant stratum corneum barrier function associated with pyoderma, as well as the involvement of staphylococcal exfoliative toxins in the pathogenesis of pyoderma.
2. Aberrant stratum corneum barrier function in atopic dermatitis.
Atopic dermatitis is a clonic, relapsing pruritic skin disease recognized in humans and dogs. Our laboratory is investigating abnormal expression of the molecules associated with stratum corneum barrier function in canine atopic dermatitis and its involvement in the disease.
Reference articles and patents
1. Yoon JS, Nishifuji K, Ishioroshi S, Ide K, Iwasaki T.Skin lipid profiling in normal and seborrhoeic shih tzu dogs.Vet Dermatol. 2013; 24(1):84-89.
2. Nishifuji K, Yoon JS. The stratum corneum: the rampart of the mammalian body.Vet Dermatol. 2013; 24(1):60-72.
3. Kanda S, Sasaki T, Shiohama A, Nishifuji K, Amagai M, Iwasaki T, Kudoh J. Characterization of canine filaggrin: gene structure and protein expression in dog skin.Vet Dermatol. 2013; 24(1):25-31.
4. Yoon JS, Nishifuji K, Sasaki A, Ide K, Ishikawa J, Yoshihara T, Iwasaki T. Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis.Exp Dermatol. 2011; 20(9):732-736.
5. Iyori K, Futagawa-Saito K, Hisatsune J, Yamamoto M, Sekiguchi M, Ide K, Son WG, Olivry T, Sugai M, Fukuyasu T, Iwasaki T, Nishifuji K. Staphylococcus pseudintermedius exfoliative toxin EXI selectively digests canine desmoglein 1 and causes subcorneal clefts in canine epidermis.Vet Dermatol. 2011; 22(4):319-326.
6. Iyori K, Hisatsune J, Kawakami T, Shibata S, Murayama N, Ide K, Nagata M, Fukata T, Iwasaki T, Oshima K, Hattori M, Sugai M, Nishifuji K. Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs.FEMS Microbiol Lett. 2010; 312(2):169-175.
7. Shimada K, Yoon JS, Yoshihara T, Iwasaki T, Nishifuji K. Increased transepidermal water loss and decreased ceramide content in lesional and non-lesional skin of dogs with atopic dermatitis.Vet Dermatol. 2009; 20(5-6):541-546.
8. Nishifuji K, Sugai M, Amagai M. Staphylococcal exfoliative toxins: "molecular scissors" of bacteria that attack the cutaneous defense barrier in mammals.J Dermatol Sci. 2008; 49(1):21-31.
Contact
University Research Administration Center(URAC),
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
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