Oxygenation reaction using cytochrome P450 as a catalyst
Research members： Nobuhumi Nakamura PhD., Hiroyuki Ohno PhD.
Research fields： Applied chemistry, Biomolecular science
Departments： Institute of Engineering
Keywords： Oxygenation,cytochrome P450,hydrogen peroxide
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Summary
Cytochrome P450s are heme-thiolate enzymes found in organisms from all domains of life. This versatility makes P450 enzymes potentially useful catalysts for biosensors, biomedical devices, and bioreactors. However, there are mainly two difficulties for the application of P450s to biodevices. (i)The P450 enzymes are relatively unstable. (ii)The monooxygenation reaction requires specific redox partners and electron donors such as NADH. We study on the improvement of the electron donating system for thermophilic cytochrome P450 and additionally, the solubilization of cytochrome P450 in organic solvents to enhance reactivity.
Reference articles and patents
Y. Oku, A. Ohtaki, S. Kamitori, N. Nakamura, M. Yohda, H. Ohno, and Y. Kawarabayasi, J. Inorg. Biochem., 98, 1194-1199 (2004).
H. Matsumura, N. Nakamura, M. Yohda, and H. Ohno, Electrochem. Commun., 9, 361-364 (2007).
H. Matsumura, M. Wakatabi, S. Omi, A. Ohtaki, N. Nakamura, M. Yohda, and H. Ohno, Biochemistry, 47, 4834-4842 (2008).
H. Matsumura, K. Matsuda, N. Nakamura, A. Ohtaki, H. Yoshida, S. Kamitori, M. Yohda, and H. Ohno, Metallomics, 3, 389-395 (2011).
S. Hayakawa, H. Matsumura, N. Nakamura, M. Yohda, and H. Ohno, FEBS Lett., 587, 64-97 (2013).
Contact
University Research Administration Center(URAC),
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
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